MACHEREY-NAGEL - Plasmid DNA

Molecular biology- / sequencing-grade plasmid DNA

Format	Typical yield	Product
	Single prep (silica-membrane technology)

	Mini spin columns	25–45 μg	NucleoSpin® Plasmid / Plasmid (NoLid)
	Mini spin columns	15–30 μg	NucleoSpin® Plasmid EasyPure
	Manual and automated high throughput (silica-membrane technology)

	8-well strips	4–6 μg (1 mL E. coli culture, high copy)	NucleoSpin® 8 Plasmid
	8-well strips		NucleoSpin® 8 Plasmid Core Kit

	96-well plates	4–6 μg (1 mL E. coli culture, high copy)	NucleoSpin® 96 Plasmid
			NucleoSpin® 96 Plasmid Core Kit

	96-well filter plates (filtration and precipitation)	8 μg (1.3 mL E. coli culture, high copy) 1 μg (1.3 mL E. coli culture, BAC)	NucleoSpin® 96 Flash

Transfection-grade plasmid DNA

Format	Typical yield	Product
High-/low-copy plasmid DNA, single prep (anion-exchange chromatography)

	NucleoBond® Xtra
	Midi gravity-flow columns	400 μg	NucleoBond® Xtra Midi / Midi Plus
	Maxi gravity-flow columns	1000 μg	NucleoBond® Xtra Maxi / Maxi Plus
	NucleoBond®
	Mini gravity-flow columns	20 μg	NucleoBond® PC 20
	Midi gravity-flow columns	100 μg	NucleoBond® PC 100
	Maxi gravity-flow columns	500 μg	NucleoBond® PC 500
	Mega gravity-flow columns	2000 μg	NucleoBond® PC 2000
	Giga gravity-flow columns	10000 μg	NucleoBond® PC 10000
	Large constructs, e.g., BACs, PACs, P1s, cosmids, single prep (anion-exchange chromatography)

	NucleoBond® Xtra
	Maxi gravity-flow columns	10–150 μg	NucleoBond® Xtra BAC
	NucleoBond®
	Maxi gravity-flow columns	10–100 μg	NucleoBond® BAC 100
	High-copy plasmid DNA, single prep (silica-membrane technology)

	NucleoSpin®
	Mini spin columns	15–30 μg	NucleoSpin® Plasmid Transfection-grade
	NucleoSnap
	Snap-off columns, vacuum processing	400–700 μg	NucleoSnap® Plasmid Midi
	High-copy plasmid DNA, manual and automated high throughput (silica-membrane technology)

	NucleoSpin®
	96-well plates	5–20 μg	NucleoSpin® 96 Plasmid Transfection- grade

Endotoxin-free plasmid DNA

Format	Typical yield	Product
	Plasmid DNA for transfection of endotoxin-sensitive cells (anion-exchange chromatography)

	NucleoBond® Xtra EF
	Midi gravity-flow columns	400 μg	NucleoBond® Xtra Midi EF/ Midi Plus EF
	Maxi gravity-flow columns	1000 μg	NucleoBond® Xtra Maxi EF/ Maxi Plus EF

	96-well plates	2–50 μg*	NucleoBond® 96 Xtra EF


	NucleoBond® EF
	Maxi gravity-flow columns	500 μg	NucleoBond® PC 500 EF
	Mega gravity-flow columns	2000 μg	NucleoBond® PC 2000 EF
	Giga gravity-flow columns	10000 μg	NucleoBond® PC 10000 EF
	Preparative scale columns compatible with FPLC, HPLC, and Bio Pilot systems		NucleoBond® PC Prep 100

* Depending on cell cultivation

Plasmid DNA concentration and desalting

Format	Typical yield	Product
Plasmid DNA concentration and desalting

	Desalting tool	500 µg	NucleoBond® Finalizer
	Desalting tool	2000 μg	NucleoBond® Finalizer Large
	Desalting tool		NucleoSpin® Finisher Midi

	Desalting tool		NucleoSnap® Finisher Midi
	Desalting tool		NucleoSnap® Finisher Maxi