MACHEREY-NAGEL - RNA

RNA from cells and tissue

Format	Sample	Product
Single prep (silica-membrane technology)

	Mini spin columns	< 30 mg tissue, < 1 x 107 cultured cells	NucleoSpin® RNA Plus

	Mini spin columns	< 30 mg tissue, < 1 x 106 cultured cells	NucleoSpin® RNA

	Reagent	< 100 mg tissue, < 1 x 107 cultured cells, < 0.4 mL (viral) fluids	NucleoZOL

	Column and buffer set	< 500 µL NucleoZOL sample	NucleoSpin® RNA Set for NucleoZOL

	XS spin columns	< 5 mg tissue, 1–105 cultured cells	NucleoSpin® RNA XS

	Midi spin columns	< 200 mg tissue, < 5 x 107 cultured cells	NucleoSpin® RNA Midi
	Gravity-flow columns (anion-exchange chromatography)	< 100 mg tissue, < 2 x 107 cultured cells	NucleoBond® RNA/DNA
	Manual and automated high throughput (HTP)

	8-well strips (silica-membrane technology)	< 20 mg tissue, < 107 cultured cells	NucleoSpin® 8 RNA NucleoSpin® 8 RNA Core Kit

	96-well plates (silica-membrane technology)	< 20 mg tissue, < 107 cultured cells	NucleoSpin® 96 RNA NucleoSpin® 96 RNA Core Kit

	Flexible (magnetic-bead technology)	< 20 mg tissue, < 2 x 106 cultured cells	NucleoMag® RNA

MicroRNA

Format	Sample	Product
Single prep (silica-membrane technology)

Mini spin columns	< 107 cultured cells, < 30 mg tissue, < 50 mg plant tissue, < 150 μL reaction mixture	NucleoSpin® miRNA

Mini spin columns	< 300 µL plasma, serum (< 900 µLwith multiple loading steps)	NucleoSpin® miRNA Plasma

Precpitation

Solution	serum, plasma	Exosome Precipitation Solution (Serum/Plasma)

Solution	urine	Exosome Precipitation Solution (Urine)

RNA from blood

Format	Sample	Product
	Single prep (silica-membrane technology)

	Mini spin columns	< 200 µL whole blood (< 400 µL with two loading steps)	NucleoSpin® RNA Blood

	Midi spin columns	400–1300 µL whole blood	NucleoSpin® RNA Blood Midi
	Manual and automated high throughput (HTP)

	8-well strips (silica-membrane technology)	< 400 µL whole blood	NucleoSpin® 8 RNA Blood

	96-well plates (silica-membrane technology)	< 400 µL whole blood	NucleoSpin® 96 RNA Blood

Total RNA from FFPE samples

Format	Sample	Product
	Single prep (silica-membrane technology)

	Mini spin columns	4–10 sections (á 10 μm, á up to 6 cm2 tissue area), < 50 mg of tissue	NucleoSpin® totalRNA FFPE

	XS spin columns	1–3 sections (á 10 μm, á up to 6 cm2 tissue area), < 5 mg of tissue	NucleoSpin® totalRNA FFPE XS

RNA from plant

Format	Sample	Product
	Single prep (silica-membrane technology)

	Mini spin columns	< 500 mg plant / fungal material	NucleoSpin® RNA Plant and Fungi

	Mini spin columns	< 100 mg (wet weight), < 20 mg (dry weight) plant tissue	NucleoSpin® RNA Plant

Poly(A) mRNA from total RNA

Format	Sample	Product
	Single prep (affinity chromatography)

	Latex beads	250–1000 μg total RNA	NucleoTrap® mRNA

RNA clean-up

ormat	Sample	Product
	Single prep (silica-membrane technology)

	Mini spin columns	< 200 μL phenol / chloroform extract or reaction mixture	NucleoSpin® RNA Clean-up

	Mini spin columns – XS design	< 300 μL RNA solution containing < 90 μg RNA	NucleoSpin® RNA Clean-up XS

RNA, DNA, and protein

Format	Sample	Product
	Single prep (silica-membrane technology)

	Total RNA, DNA, and protein isolation
	Mini spin columns	< 30 mg tissue, < 100 mg plant tissue, < 5 x 106 cultured cells	NucleoSpin® TriPrep

	Total RNA and protein isolation
	Mini spin columns	< 30 mg tissue, < 100 mg plant tissue, < 5 x 106 cultured cells	NucleoSpin® RNA/Protein

	Total RNA and DNA isolation
	Buffer set	< 30 mg tissue, < 100 mg plant tissue, < 5 x 106 cultured cells	NucleoSpin® RNA/DNA Buffer Set

	Protein quantification
	Buffer and reagent set	Protein dissolved in Protein Solving Buffer PSB or Laemmli Buffer	Protein Quantification Assay